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KMID : 0545120140240040489
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 4 p.489 ~ p.496
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Purification and Characterization of a Thermostable Xylanase from Paenibacillus sp. NF1 and its Application in Xylooligosaccharides Production
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Hong-chen Zheng
Ming-zhe Sun
Ling-cai Meng
Hai-sheng Pei
Xiu-qing Zhang
Zheng Yan
Wen-hui Zeng
Jing-sheng Zhang
Jin-rong Hu
Fu-ping Lu
Jun-she Sun
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Abstract
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High levels of extracellular xylanase activity (211.79 IU/mg) produced by Paenibacillus sp. NF1 were detected when it was submerged-cultured. After three consecutive purification steps using Octyl-Sepharose, Sephadex G75, and Q-Sepharose columns, a thermostable xylanase (XynNF) was purified to homogeneity and showed a molecular mass of 37 kDa according to SDS-PAGE. The specific activity of the purified XynNF was up to 3,081.05 IU/mg with a 14.55-fold purification. The activity of XynNF was stimulated by Ca2+, Ba2+, DTT, and β-mercaptoethanol, but was inhibited by Fe3+, Zn2+, Fe2+, Cu2+, SDS, and EDTA. The purified XynNF displayed a greater affinity for oat spelt xylan with the maximal enzymatic activity at 60°C and pH 6.0. XynNF, which was shown to be cellulose-free, with high stability at high temperature (70°C-80°C) and low pH range (pH 4.0-7.0), is potentially valuable for various industrial applications. The end products of high efficient oat spelt xylan hydrolysis by XynNF (an endoxylanase) containing 95.8% xylooligosaccharides of 2-4 degree of polymerization (DP2-4) with the enrichment of xylobiose (61.5%) indicated that XynNF is a promising candidate for xylooligosaccharides production.
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KEYWORD
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Endoxylanase, Paenibacillus sp, Purification, Characterization, Oat spelt xylan, Xylooligosaccharides
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